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The second phase of the demonstration project evaluates the performance of diploid and triploid oysters planted in early spring, as opposed to the first phase in which oysters were planted in late summer. In addition to seed provided to participants in March (see previous article), oysters of each ploidy type were field nursed by the UF project team at their experimental lease off Cedar Key in April. At that time, diploids were 29 mm in shell height (SH) and triploids were 34 mm. Oysters were stocked into 9 mm mesh Vexar bags at densities of 700 per bag. After seven

Oysters typically acquire their flavor from their growing environment and are frequently named after their harvest location. Consumers can expect variations in appearance, color, aroma, taste, and mouth-feel that influence preferences, value, and reputation. Sensory attributes and acceptability can make the difference in market success of Florida cultured oysters. Diploid and triploid oysters harvested from the UF demonstration site in Cedar Key were delivered under refrigerated conditions to the UF/IFAS Food Science and Human Nutrition Department in Gainesville on May 24, 2017 for evaluation under the guidance of Dr. Charles Sims. All rules pertaining to harvesting and handling of oysters,

To quantify the effects of seasonal harvests on ploidy type, several spawns using tetraploid oysters held from the spring 2016 spawn were attempted in the fall at a participating commercial hatchery; however, viable gametes were not obtained. Since availability of triploid seed was limited, it was decided to purchase single-set seed with project funds from the Louisiana Sea Grant oyster hatchery and Auburn University shellfish hatchery. Disease certifications and ploidy verification required by the Department of Agriculture and Consumer Services for out-of-state oyster seed were obtained. These stocks were combined. A similar number of diploid seed was obtained from a

Six months after seed oysters (average 25 mm in shell height) were stocked into 14 mm mesh Vexar bags (October 2016), they were harvested in April 2017 (12 months from spawn). Replicated field trials were conducted at the UF experimental lease within the Dog Island Aquaculture Use Area off Cedar Key to document the effects of ploidy (diploid versus triploid), stocking density (125, 150, 175 oysters/bag), and float design (square versus bullet) on oyster production. To determine growth, a sample of 35 oysters from each of the 42 bags (six replicate bags per treatment) were measured for shell height, length,

Ten growers in four west coast counties (Charlotte, Franklin, Levy, and Wakulla) participating in this project received oyster seed (2500 of each ploidy type, 20-22 mm in shell height) during July 2016 to grow on their leases. These growers used a variety of culture methods, which allowed for evaluation of site and gear interaction on ploidy type. To document production performance and assess health of diploid (2N) and triploid (3N) oysters under commercial conditions, oysters from three replicate bags (four baskets if using the adjustable longline system) for each ploidy type were provided to the UF project team at harvest.

A similar number of oysters provided to project participants were also cultured at the UF experimental lease off Cedar Key so that growth and survival could be documented bimonthly during growout. Oysters (ave 26 mm shell length) were stocked in 14 mm mesh bags in October (see previous post) and sampled in December 2016 and, again, in February 2017. Three bags from each of the density and ploidy combinations, a total of 18 bags, were sampled each period. These bags were supported with square floats; in addition, three bags supported with bullet floats were also sampled. Different replicate bags were